| 货号 | 96687S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Monkey |
| 应用 | WB,IP,CHIP |
| 目标/特异性 | SRC-2 (D2X4M) Rabbit mAb recognizes endogenous levels of total SRC-2 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) Chromatin IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | There are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2/GRIP1/NCoA-2), and SRC-3 (ACTR/pCIP/RAC3/TRAM-1/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1/PAX3 and SRC-2/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
| 参考文献 | 1 . Giraud, S. et al. (2002) J. Biol. Chem. 277, 8004-8011. 2 . Na, S.Y. et al. (1998) J. Biol. Chem. 273, 10831-10834. 3 . Louie, M.C. et al. (2004) Mol. Cell Biol. 24, 5157-5171. 4 . Lee, S.K. et al. (1999) Mol. Endocrinol. 13, 1924-1933. 5 . Spencer, T.E. et al. (1997) Nature 389, 194-198. 6 . Chen, H. et al. (1997) Cell 90, 569-580. 7 . Koh, S.S. et al. (2001) J. Biol. Chem. 276, 1089-1098. 8 . Chen, D. et al. (1999) Science 284, 2174-2177. 9 . Wu, R.C. et al. (2004) Mol. Cell 15, 937-949. 10 . Rowan, B.G. et al. (2000) J. Biol. Chem. 275, 4475-4483. 11 . Zhou, H.J. et al. (2005) Cancer Res. 65, 7976-7983. 12 . Torres-Arzayus, M.I. et al. (2004) Cancer Cell 6, 263-274. 13 . Wachtel, M. et al. (2004) Cancer Res. 64, 5539-5545. 14 . Deguchi, K. et al. (2003) Cancer Cell 3, 259-271. |
Immunoprecipitation of SRC-2 from LnCAP cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SRC-2 (D2X4M) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d, followed by treatment with β-estradiol (10 nM, 45 min) and SRC-2 (D2X4M) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 5 ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. The figure shows binding across NRIP1, a known target gene of SRC-2 (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet. | |
Western blot analysis of extracts from various cell lines using SRC-2 (D2X4M) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d, followed by treatment with β-estradiol (10 nM, 45 min) and either SRC-2 (D2X4M) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human NRIP1 Upstream Primers #35998, human XBP1 upstream primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
