| 货号 | 95473S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB |
| 目标/特异性 | OMA1 (D4J7K) Rabbit mAb recognizes endogenous levels of total OMA1 protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mitochondria continuously divide and fuse. This dynamic process is highly regulated in response to various physiological cues (1,2). The GTPase OPA1 mediates the fusion of the mitochondrial inner membrane. Constitutive proteolytic processes mediated by OMA1 (S1 site) and YME1L (S2 site) convert long isoforms (L-OPA1) into short isforms (S-OPA1). The balance between L-OPA1 and S-OPA1 is required to maintain a normal morphology of mitochondria (3,4). OMA1 is synthesized as a precursor and processed into a mature form (5,6). OMA1 is constitutively active and cleaves L-OPA1 at the S1 site. However, various stress stimuli can further activate OMA1 and result in the rapid and complete conversion of L-OPA1 into S-OPA1, which inhibits fusion and causes mitochondrial fragmentation (7). |
| 存放说明 | -20C |
| 计算分子量 | 37 |
| 参考文献 | 1 . Chan, D.C. (2012) Annu Rev Genet 46, 265-87. 2 . Lee, H. and Yoon, Y. (2016) Biochem Soc Trans 44, 1725-1735. 3 . Anand, R. et al. (2014) J Cell Biol 204, 919-29. 4 . MacVicar, T. and Langer, T. (2016) J Cell Sci 129, 2297-306. 5 . Head, B. et al. (2009) J Cell Biol 187, 959-66. 6 . Baker, M.J. et al. (2014) EMBO J 33, 578-93. 7 . Anand, R. et al. (2014) J Cell Biol 204, 919-29. |
Western blot analysis of extracts from various cell lines using OMA1 (D4J7K) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Mature OMA1 is smaller than its precursor, which has a calculated molecular weight of 59 kDa. |
