| 货号 | 16416S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IP |
| 目标/特异性 | HUS1 (D4J9H) Rabbit mAb recognizes endogenous levels of total HUS1 protein. In some cell lysates, this antibody detects a 45 kDa band of unknown origin. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | DNA damage resulting from genotoxic stress activates cellular checkpoints that prevent or delay cell division until damaged DNA is repaired or the cell follows an apoptotic pathway. The Rad9 homolog A (Rad9A, Rad9) protein is part of a checkpoint protein complex that acts as an early sensor of DNA damage. Together with the HUS1 and Rad1 checkpoint proteins, Rad9 forms a heterotrimeric 9-1-1 complex with a ring structure similar to the processivity factor PCNA. The 9-1-1 complex induces multiple signaling pathways, including the ATM- and ATR-activated DNA repair pathways (1,2). A functional 9-1-1 complex is required for ATR-dependent S phase checkpoint signaling (3). The 9-1-1 complex interacts with DNA topoisomerase 2-binding protein 1 (TopBP1) in response to DNA damage, activating ATR and causing signal amplification through further recruitment of TopBP1 (4). The 9-1-1 complex interacts with DNA mismatch repair proteins MSH2, MSH3, and MSH6 to play a role in mismatch repair (5). During an error-free DNA damage tolerance process, the 9-1-1 complex cooperates with polyubiquitinated PCNA and Exo1 nuclease to support switching of the replicative polymerase to the undamaged template (6). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
| 参考文献 | 1 . Broustas, C.G. and Lieberman, H.B. (2012) J Cell Biochem 113, 742-51. 2 . Kai, M. (2013) Biomolecules 3, 75-84. 3 . Bao, S. et al. (2004) Oncogene 23, 5586-93. 4 . Ohashi, E. et al. (2014) DNA Repair (Amst) 21, 1-11. 5 . Bai, H. et al. (2010) DNA Repair (Amst) 9, 478-87. 6 . Karras, G.I. et al. (2013) Mol Cell 49, 536-46. |
Western blot analysis of extracts from various cell lines using HUS1 (D4J9H) Rabbit mAb. | |
Immunoprecipitation of HUS1 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is HUS1 (D4J9H) Rabbit mAb. Western blot analysis was performed using HUS1 (D4J9H) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody. |
