| 货号 | 40868S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IHC |
| 目标/特异性 | CD40 (D8W3N) Rabbit mAb recognizes endogenous levels of total CD40 protein. |
| 使用方法 | Western Blotting (1:1000) IHC-Leica® Bond™ (1:200) Immunohistochemistry (Paraffin) (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), is a type I transmembrane protein expressed on the surface of B cells and professional antigen-presenting cells of the immune system, as well as on several non-hematopoietic cell types and cancers (1-4). CD40 interacts with CD40 ligand (CD40L/TNFSF5), which is expressed primarily on activated T cells but has also been reported on blood platelets, mast cells, basophils, NK cells, and B cells (5). Upon engagement with CD40L, CD40 signals through TNF receptor associated factors and MAP kinase signaling pathways, resulting in a wide variety of immune and inflammatory responses, including dendritic cell activation and cross-presentation, T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation (6-8). The CD40/CD40L axis is essential for the initiation and progression of cellular and humoral adaptive immunity, and is an important area of interest in the study of tumor immunology, neurodegenerative diseases, vascular diseases, and inflammatory disorders (9-12). |
| 存放说明 | -20C |
| 计算分子量 | 42 |
| 参考文献 | 1 . Banchereau, J. et al. (1995) Adv Exp Med Biol 378, 79-83. 2 . Cooke, P.W. et al. (1999) J Pathol 188, 38-43. 3 . Hakkarainen, T. et al. (2003) Clin Cancer Res 9, 619-24. 4 . Slobodova, Z. et al. (2011) Neoplasma 58, 189-97. 5 . Gauchat, J.F. et al. (1993) FEBS Lett 315, 259-66. 6 . Quezada, S.A. et al. (2004) Annu Rev Immunol 22, 307-28. 7 . Spriggs, M.K. et al. (1992) J Exp Med 176, 1543-50. 8 . Elgueta, R. et al. (2009) Immunol Rev 229, 152-72. 9 . Korniluk, A. et al. (2014) Tumour Biol 35, 9447-57. 10 . Townsend, K.P. et al. (2005) Eur J Immunol 35, 901-10. 11 . Hassan, G.S. et al. (2012) Immunobiology 217, 521-32. 12 . Zhang, B. et al. (2013) Immunol Lett 153, 58-61. |
Western blot analysis of extracts from various cell lines and tissues using CD40 (D8W3N) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 8hr; +), using CD40 (D8W3N) Rabbit mAb (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). | |
Imunohistochemical analysis of paraffin-embedded human infiltrating ductal carcinoma of the breast using CD40 (D8W3N) Rabbit mAb performed on the Leica® BOND™ Rx. | |
Imunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD40 (D8W3N) Rabbit mAb. | |
Imunohistochemical analysis of paraffin-embedded human Non-Hodgkins lymphoma using CD40 (D8W3N) Rabbit mAb. | |
Imunohistochemical analysis of paraffin-embedded RL-7 cell pellets (left, positive) or MCF7 cell pellets (right, negative) using CD40 (D8W3N) Rabbit mAb. | |
Imunohistochemical analysis of paraffin-embedded human tonsil using CD40 (D8W3N) Rabbit mAb. |
