| 货号 | 39036S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | WB,IP,IF,F |
| 目标/特异性 | VCAM-1 (D8U5V) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total VCAM-1 protein. This antibody recognizes both long and short isoforms of VCAM-1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) Immunofluorescence (Frozen) (1:400) Immunofluorescence (Immunocytochemistry) (1:400) Flow Cytometry (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3). |
| 存放说明 | -20C |
| 计算分子量 | 35-45, 85-100 |
| 参考文献 | 1 . Osborn, L. et al. (1989) Cell 59, 1203-11. 2 . Hession, C. et al. (1991) J Biol Chem 266, 6682-5. 3 . Elices, M.J. et al. (1990) Cell 60, 577-84. |
Western blot analysis of extracts from various cell lines using VCAM-1 (D8U5V) Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of VCAM-1 protein from MS-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is VCAM-1 (D8U5V) Rabbit mAb (Mouse Specific). | |
Confocal immunofluorescent analysis of mouse small intestine using VCAM-1 (D8U5V) Rabbit mAb (Mouse Specific) (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of mouse spleen at 20X magnification (left) and 60X magnification (right) using VCAM-1 (D8U5V) Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of MS-1 (left, positive) and Raw 264.7 (right, negative) cells using VCAM-1 (D8U5V) Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of MS-1 cells (green) and A20 cells (blue) using VCAM-1 (D8U5V) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab)2 Fragments (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
