| 货号 | 17700S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | Complexin-1 (D5Q5H) Rabbit mAb recognizes endogenous levels of total Compexin-1 protein. This antibody does not cross-react with Complexin-2. |
| 使用方法 | W IP IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses. |
| 存放说明 | -20C |
| 计算分子量 | 14 |
| 参考文献 | 1 . Chen, X. et al. (2002) Neuron 33, 397-409. 2 . McMahon, H.T. et al. (1995) Cell 83, 111-9. 3 . Reim, K. et al. (2005) J Cell Biol 169, 669-80. 4 . Reim, K. et al. (2001) Cell 104, 71-81. 5 . Huntwork, S. and Littleton, J.T. (2007) Nat Neurosci 10, 1235-7. 6 . Maximov, A. et al. (2009) Science 323, 516-21. 7 . Xue, M. et al. (2008) Proc Natl Acad Sci U S A 105, 7875-80. |
Confocal immunofluorescent analysis of Neuro-2a (positive, left panels) and NIH/3T3 (negative, right panels) using Complexin-1 (D5Q5H) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red, upper panels). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cells lines and tissues using Complexin-1 (D5Q5H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full length human complexin-1 (hCPLX-1-Myc; +) or complexin-2 (hCPLX-2-Myc; +), using Complexin-1 (D5Q5H) Rabbit mAb (upper), and Myc-Tag (71D10) Rabbit mAb #2278 (lower). | |
Immunoprecipitation of Complexin-1 from Neuro-2a cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 and lane 3 is Complexin-1 (D5Q5H) Rabbit mAb. Western blot was performed using Complexin-1 (D5Q5H) Rabbit mAb. |
