| 货号 | 18215S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | BRCC36 (D5E5H) Rabbit mAb recognizes endogenous levels of total BRCC36 protein. This antibody detects BRCC36 isoform1 and BRCC36 isoform2. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, UCH, and JAMM/MPN. BRCC36 is a zinc-dependent DUB belonging to the JAMM/MPN subfamily and participates in DNA damage responses and interferon signaling by specifically catalyzing hydrolysis of K63-linked polyubiquitin chains (3,4). In the nucleus, BRCC36 is part of the BRCA1-A complex that contains RAP80, BRCA1, ABRAXAS, and MERIT40 (5,6). This complex plays a critical role in mediating the cellular repair of DNA double strand breaks induced by ionizing radiation (7-10). Research studies have shown that BRCC36 is overexpressed in a high percentage of breast tumors, which may contribute to resistance of breast cancer cells to ionizing radiation-induced apoptosis (4). BRCC36 also functions in the cytoplasm as part of a distinct complex known as the BRCC36-containing isopeptide complex (BRISC) (5,6). Indeed, research studies have shown that BRCC36 deubquitinates and stabilizes IFNAR1 to modulate the cellular response to interferons (3). |
| 存放说明 | -20C |
| 计算分子量 | 33, 40 |
| 参考文献 | 1 . Nijman, S.M. et al. (2005) Cell 123, 773-86. 2 . Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3 . Zheng, H. et al. (2013) Cell Rep 5, 180-93. 4 . Chen, X. et al. (2006) Cancer Res 66, 5039-46. 5 . Feng, L. et al. (2010) J Biol Chem 285, 30982-8. 6 . Patterson-Fortin, J. et al. (2010) J Biol Chem 285, 30971-81. 7 . Shao, G. et al. (2009) Proc Natl Acad Sci U S A 106, 3166-71. 8 . Wang, B. and Elledge, S.J. (2007) Proc Natl Acad Sci U S A 104, 20759-63. 9 . Sobhian, B. et al. (2007) Science 316, 1198-202. 10 . Wang, B. et al. (2009) Genes Dev 23, 729-39. |
Western blot analysis of extracts from various cell lines using BRCC36 (D5E5H) Rabbit mAb. | |
Immunoprecipitation of BRCC36 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BRCC36 (D5E5H) Rabbit mAb. Western blot analysis was performed using BRCC36 (D5E5H) Rabbit mAb. Anti-Rabbit HRP-conjugated light chain-specific secondary antibody was used for detection. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human BRCC36 isoform 2 (hBRCC36 iso2-Myc/DDK; +) and isoform 1 (hBRCC36 iso1-Myc/DDK; +), using BRCC36 (D5E5H) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower). |
