| 货号 | 83630S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human |
| 应用 | IHC |
| 目标/特异性 | p27 Kip1 (SX53G8.5) Mouse mAb (IHC Formulated) recognizes endogenous levels of total p27 Kip1 protein. |
| 使用方法 | Immunohistochemistry (Paraffin) (1:700) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4). |
| 存放说明 | -20C |
| 参考文献 | 1 . Lloyd, R.V. et al. (1999) Am J Pathol 154, 313-23. 2 . Polyak, K. et al. (1994) Genes Dev 8, 9-22. 3 . Kato, J.Y. et al. (1994) Cell 79, 487-96. 4 . Vlach, J. et al. (1997) EMBO J 16, 5334-44. |
Immunohistochemical analysis of paraffin-embedded U266 cell pellets (left, high-expressing) and Daudi (right, low-expressing) cell pellets using p27 Kip1 (SX53G8.5) Mouse mAb (IHC Formulated). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p27 Kip1 (SX53G8.5) Mouse mAb (IHC Formulated). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using p27 Kip1 (SX53G8.5) Mouse mAb (IHC Formulated). | |
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using p27 Kip1 (SX53G8.5) Mouse mAb (IHC Formulated). | |
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkins lymphoma using p27 Kip1 (SX53G8.5) Mouse mAb (IHC Formulated). |
