| 货号 | 81254S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB,IHC |
| 目标/特异性 | PVR/CD155 (D8A5G) Rabbit mAb recognizes endogenous levels of total CD155 protein. |
| 使用方法 | Western Blotting (1:1000) IHC-Leica® Bond™ (1:200) Immunohistochemistry (Paraffin) (1:200) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Poliovirus receptor (PVR, CD155) is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. Also known as nectin-like 5 (Necl-5), PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells (1,2). Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells (3). Additional research indicates that PVR (CD155, Necl-5) expression may play a role in invasiveness of lung adenocarcinoma (4,5). In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity (6). |
| 存放说明 | -20C |
| 计算分子量 | 60-80 |
| 参考文献 | 1 . Takai, Y. et al. (2008) Nat Rev Mol Cell Biol 9, 603-15. 2 . Sakisaka, T. et al. (2007) Curr Opin Cell Biol 19, 593-602. 3 . Minami, Y. et al. (2007) Biochem Biophys Res Commun 352, 856-60. 4 . Tane, S. et al. (2013) Exp Mol Pathol 94, 330-5. 5 . Nakai, R. et al. (2010) Cancer Sci 101, 1326-30. 6 . Stanietsky, N. and Mandelboim, O. (2010) FEBS Lett 584, 4895-900. |
Western blot analysis of extracts from various cell lines using PVR/CD155 (D8A5G) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using PVR/CD155 (D8A5G) Rabbit mAb performed on the Leica® Bond™ Rx. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PVR/CD155 (D8A5G) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded A549 cell pellet (left, positive) and Daudi cell pellet (right, negative) using PVR/CD155 (D8A5G) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using PVR/CD155 (D8A5G) Rabbit mAb. |
