| 货号 | 20808S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Cyclin E1 (D7T3U) Rabbit mAb recognizes endogenous levels of total cyclin E1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of cyclin E1. This antibody does not cross-react with cyclin E2. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 48 |
| 参考文献 | 1 . Lauper, N. et al. (1998) Oncogene 17, 2637-43. 2 . Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. 3 . Ewen, M.E. (2000) Genes Dev 14, 2265-70. 4 . Won, K.A. and Reed, S.I. (1996) EMBO J 15, 4182-93. 5 . Koepp, D.M. et al. (2001) Science 294, 173-7. 6 . Welcker, M. et al. (2003) Mol Cell 12, 381-92. 7 . Ye, X. et al. (2004) J Biol Chem 279, 50110-9. |
Western blot analysis of extracts from various cell lines using Cyclin E1 (D7T3U) Rabbit mAb. | |
Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with aphidicolin (10 μg/ml; 24 hr; +) using Cyclin E1 (D7T3U) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
