| 货号 | 23328S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | FGF Receptor 2 (D4L2V) Rabbit mAb recognizes endogenous levels of total FGFR2 protein. |
| 使用方法 | W IP IHC-P IF-IC F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 92,145 |
| 参考文献 | 1 . Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97. 2 . Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8. 3 . Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89. 4 . Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78. 5 . Larsson, H. et al. (1999) J Biol Chem 274, 25726-34. 6 . Muh, S.J. et al. (2002) J Biol Chem 277, 50143-54. 7 . Coutts, J.C. and Gallagher, J.T. (1995) Immunol Cell Biol 73, 584-9. 8 . Jeftha, A. et al. (2004) J Clin Pediatr Dent 28, 173-6. 9 . Wilkinson, C.C. et al. (2012) Childs Nerv Syst 28, 1221-6. 10 . Slavotinek, A. et al. (2009) Am J Med Genet A 149A, 1814-7. 11 . Katoh, M. (2009) J Invest Dermatol 129, 1861-7. |
Flow cytometric analysis of SW620 (blue) and KATO III (green) cells using FGF Receptor 2 (D4L2V) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of KATO III (high expressing; left), AN3 CA (low expressing; center), and SW620 (negative; right) cells using FGF Receptor 2 (D4L2V) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of FGFR2 protein from Saos-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FGF Receptor 2 (D4L2V) Rabbit mAb. Western blot analysis was performed using FGF Receptor 2 (D4L2V) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded KATO III (high expressing; left), AN3 CA (low expressing; center), and SW620 (negative; right) cell pellets using FGF Receptor 2 (D4L2V) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FGF Receptor 2 (D4L2V) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using FGF Receptor 2 (D4L2V) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FGF Receptor 2 (D4L2V) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FGF Receptor 2 (D4L2V) Rabbit mAb. |
