| 货号 | 28409S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | TAF15 (D8V6Q) Rabbit mAb recognizes endogenous levels of total TAF15 protein. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TBP binding protein associated factor 15 (TAF15) is a member of the multifunctional FET (FUS, EWS, and TAF15) family of proteins that bind both DNA and RNA and may be important for transcriptional regulation and RNA processing (1,2). Research studies show that FET family proteins, including TAF15, can be found as oncogenic fusion proteins with transcription factors in a variety of cancers. The fusion protein consists of the FET protein amino-terminal transcription activation domain and the transcription factor DNA binding domain, creating an aberrant transcription factor with potent transactivation potential (1-3). TAF15 can be part of a subset of the transcription factor IID (TFIID) complex, indicating that it may form a transcription pre-initiation complex at active genes, even if it is not considered a canonical TAF (4,5). Additional studies suggest roles for TAF15 in RNA splicing through its association with U1 small nuclear RNA and in regulation of cell cycle regulatory gene expression through a mechanism involving miRNA biosynthesis (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 77 |
| 参考文献 | 1 . Law, W.J. et al. (2006) Brief Funct Genomic Proteomic 5, 8-14. 2 . Kovar, H. (2011) Sarcoma 2011, 837474. 3 . Bertolotti, A. et al. (1999) Oncogene 18, 8000-10. 4 . Bertolotti, A. et al. (1996) EMBO J 15, 5022-31. 5 . Bertolotti, A. et al. (1998) Mol Cell Biol 18, 1489-97. 6 . Jobert, L. et al. (2009) EMBO Rep 10, 494-500. 7 . Ballarino, M. et al. (2013) Oncogene 32, 4646-55. |
Western blot analysis of extracts from various cell lines using TAF15 (D8V6Q) Rabbit mAb. | |
Immunoprecipitation of TAF15 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TAF15 (D8V6Q) Rabbit mAb. |
