| 货号 | 32346S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | SPHK2 (D2V3G) Rabbit mAb recognizes endogenous levels of total SPHK2 protein. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood. |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26. 2 . Xia, P. et al. (2000) Curr Biol 10, 1527-30. 3 . Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903. 4 . Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8. 5 . Kohama, T. et al. (1998) J Biol Chem 273, 23722-8. 6 . Liu, H. et al. (2000) J Biol Chem 275, 19513-20. 7 . Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511. 8 . Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407. 9 . Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28. 10 . Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34. 11 . Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8. 12 . Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39. |
Western blot analysis of extracts from Raji, MOLT-4, and human kidney using SPHK2 (D2V3G) Rabbit mAb. |
