| 货号 | 32720S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | CD13/APN (D6V1W) Rabbit mAb recognizes endogenous levels of total CD13/APN protein. |
| 使用方法 | W IP IHC-P IF-IC F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Aminopeptidase N (APN, CD13) is a widely expressed, membrane-bound proteolytic enzyme that breaks down peptides during digestion, cleaves cell surface antigens during antigen presentation, and acts as a receptor for human viruses, including several coronaviruses. This multifunctional protein is implicated in the regulation of many biological processes, including angiogenesis, cell proliferation, cell migration, inflammation and immune response (1,2). APN was originally identified as the cell surface antigen CD13, which is expressed in myeloid lineage hematopoietic cells and myeloid leukemia (3). Identified substrates of aminopeptidase N include the angiotensin I-III peptide hormones, the opioid peptide met-enkephalin, and cytokines MCP-1 and MIP-1 (4). Abnormal APN protein expression is seen in various forms of cancer, with high APN expression associated with poor survival in colon cancer and non-small cell lung cancer and silenced APN expression related to poor prognosis in prostate cancer (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 160 |
| 参考文献 | 1 . Luan, Y. and Xu, W. (2007) Curr Med Chem 14, 639-47. 2 . Mina-Osorio, P. (2008) Trends Mol Med 14, 361-71. 3 . Look, A.T. et al. (1989) J Clin Invest 83, 1299-307. 4 . Bauvois, B. (2004) Oncogene 23, 317-329. 5 . Hashida, H. et al. (2002) Gastroenterology 122, 376-86. 6 . Tokuhara, T. et al. (2006) Clin Cancer Res 12, 3971-8. 7 . Sørensen, K.D. et al. (2013) Br J Cancer 108, 420-8. |
Confocal immunofluorescent analysis of PANC-1 (left) or HEK293 (right) cells using CD13/APN (D6V1W) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of peripheral blood using CD13/APN (D6V1W) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (red). Anti-Rabbit IgG (H+L), F(ab)2Fragment (Alexa Fluor®488 Conjugate) #4412 was used a secondary antibody. | |
Western blot analysis of extracts from OVCAR8, SK-MEL-2, mouse kidney, and rat liver using CD13/APN (D6V1W) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Immunoprecipitation of CD13/APN from PANC-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD13/APN (D6V1W) Rabbit mAb. Western blot analysis was performed using CD13/APN (D6V1W) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD13/APN (D6V1W) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded PANC-1 (left) and HEK-293 (right) cell pellets using CD13/APN (D6V1W) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded normal human kidney using CD13/APN (D6V1W) Rabbit mAb. |
