| 货号 | 32770S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | IF-IC |
| 目标/特异性 | PHGDH (D3D5E) Rabbit mAb recognizes endogenous levels of total PHGDH protein. |
| 使用方法 | IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Mammalian cells synthesize serine de novo by diverting a portion of the glycolytic intermediate 3-phosphoglycerate into the phosphorylated pathway of serine synthesis. This shift supports anabolism by providing precursors for the biosynthesis of proteins, nucleotides, creatine, porphyrins, phospholipids, and glutathione. Phosphoglycerate dehydrogenase (PHGDH) catalyzes the first step in the serine biosynthesis pathway by converting 3-phosphoglycerate into phosphohydroxy pyruvate (1). Research studies demonstrate that an increase in serine biosynthesis supports growth and proliferation of cancer cells (2-4), which is supported by amplification and overexpression of PHGDH in a subset of melanoma and breast cancers (5,6). Suppression of PHGDH expression in cell lines with elevated PHGDH levels causes a strong decrease in cell proliferation and inhibits tumor growth in vivo (5). Additional evidence suggests that PHGDH interacts with and stabilizes FoxM1, which promotes the proliferation, invasion, and tumorigenicity of glioma cells (7). |
| 存放说明 | -20C |
| 参考文献 | 1 . Locasale, J.W. (2013) Nat Rev Cancer 13, 572-83. 2 . Amelio, I. et al. (2013) Oncogene, [Epub ahead of print]. 3 . Ma, L. et al. (2013) Cell 152, 599-611. 4 . Maddocks, O.D. et al. (2013) Nature 493, 542-6. 5 . Possemato, R. et al. (2011) Nature 476, 346-50. 6 . Locasale, J.W. et al. (2011) Nat Genet 43, 869-74. 7 . Liu, J. et al. (2013) J Neurooncol 111, 245-55. |
Confocal immunofluorescent analysis of high-expressing 293T (left), HeLa (middle), or negative 786-0 (right) cells using PHGDH (D3D5E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
