| 货号 | 40491S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-F , IF-IC |
| 目标/特异性 | M-Cadherin (D4B9L) Rabbit mAb recognizes endogenous levels of total M-Cadherin protein. The lower band in the doublet shown in C2C12 and RD extracts is unglycosylated M-Cadherin. The identity of the ~78 kDa band that is present in the C2C12 extract is not known but is likely to be a breakdown product in the lysate as it is absent in the IP input and pull-down. Note that human M-Cadherin runs slightly lower than mouse M-Cadherin. |
| 使用方法 | W IP IF-F IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35. 2 . Christofori, G. (2003) EMBO J 22, 2318-23. 3 . Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63. 4 . Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34. 5 . Rabascio, C. et al. (2004) Cancer Res 64, 4373-7. 6 . Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7. 7 . Patel, I.S. et al. (2003) Int J Cancer 106, 172-7. 8 . Sanders, D.S. et al. (2000) J Pathol 190, 526-30. 9 . Irintchev, A. et al. (1994) Dev Dyn 199, 326-37. 10 . Marti, M. et al. (2013) J Cell Sci 126, 5116-31. |
Confocal immunofluorescent analysis of C2C12 (left; positive) and 3T3 (right; negative) cells using M-Cadherin (D4B9L) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of mouse skeletal muscle using M-Cadherin (D4B9L) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (left panel, red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Image shows M-Cadherin labeling in a satellite cell on skeletal muscle fibers. | |
Western blot analysis from extracts of C2C12 and RD cell lines using M-Cadherin (D4B9L) Rabbit mAb. | |
Immunoprecipitation of M-Cadherin from C2C12 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is M-Cadherin (D4B9L) Rabbit mAb. Western blot analysis was performed using M-Cadherin (D4B9L) Rabbit mAb. |
