| 货号 | 41262S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Siglec-10 (D4E3Z) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total Siglec-10 protein. |
| 使用方法 | W IP F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Siglec-10 is a sialic acid-binding Ig-like lectin that has been shown to negatively regulate innate and adaptive immune system function (1). Siglec-10 is a type 1 transmembrane protein that has four extracellular Ig-like domains, a transmembrane region, and a cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Siglec-10 is expressed in dendritic cells (DCs), monocytes, B cells, NK cells, and T cells (2, 3). Siglec-10 interacts with CD24 to suppress immune responses to danger associated molecular patterns (DAMPs) by associating with the tyrosine phosphatase SHP-1, a negative regulator of nuclear factor-kappa B (NF-κB) (4). Siglec-10 has been shown to bind soluble CD52 leading to the impairment of phosphorylation of the T cell receptor–associated kinases Lck and Zap70 and T cell activation (3). It has been proposed that this mechanism of suppression could be involved in T cell homeostasis and the prevention of type I diabetes (3). Siglec-10 has also been identified as the leukocyte ligand for vascular adhesion protein-1 (VAP-1), which plays a key role in leukocyte trafficking (5). Lectin galactoside-binding soluble 3 binding protein (LGALS3BP), a tumor-associated immunomodulatory ligand, has also been shown to bind Siglec-10 (6). |
| 存放说明 | -20C |
| 计算分子量 | 92, 100-105 |
| 参考文献 | 1 . Crocker, P.R. et al. (2007) Nat Rev Immunol 7, 255-66. 2 . Li, N. et al. (2001) J Biol Chem 276, 28106-12. 3 . Bandala-Sanchez, E. et al. (2013) Nat Immunol 14, 741-8. 4 . Chen, G.Y. et al. (2009) Science 323, 1722-5. 5 . Kivi, E. et al. (2009) Blood 114, 5385-92. 6 . Läubli, H. et al. (2014) J Biol Chem 289, 33481-91. |
Flow cytometric analysis of 3T3 cells (blue) and A20 cells (green) using Siglec-10 (D4E3Z) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines using Siglec-10 (D4E3Z) Rabbit mAb (Mouse Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). |
