| 货号 | 43918S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | MTH1 (D6V4O) Rabbit mAb recognizes endogenous levels of total MTH1 protein. Based on the sequence of the immunogenic peptide, this antibody is expected to recognize all isoforms of MTH1. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MutT Homolog 1 (MTH1), an oxidized purine nucleoside triphosphatase, hydrolyzes potentially mutagenic oxidized nucleotide triphosphates, preventing their accumulation in nucleotide pools and their incorporation into DNA and RNA (1). In addition to its function in sanitizing the cell’s nucleotide pool, MTH1 has been shown to have anti-proliferative effects in RAS-transformed tumors (2). Researchers have shown that, while not essential in normal cells, MTH1 is required for cancer cell survival due to increased oxidative damage, and that inhibition of MTH1 activity suppresses cancer growth (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . Nakabeppu, Y. et al. (2006) DNA Repair (Amst) 5, 761-72. 2 . Giribaldi, M.G. et al. (2015) Oncotarget 6, 11519-29. 3 . Gad, H. et al. (2014) Nature 508, 215-21. 4 . Huber, K.V. et al. (2014) Nature 508, 222-7. |
Western blot analyysis of extracts from various cell lines using MTH1 (D6V4O) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immuonprecipitation of MTH1 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MTH1 (D6V4O) Rabbit mAb. Western blot analysis was performed using |
