| 货号 | 25164S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 应用 | WB |
| 目标/特异性 | MTHFR (D1E4V) Rabbit mAb recognizes endogenous levels of total MTHFR protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Methylenetetrahydrofolate reductase (MTHFR), a key enzyme in one-carbon metabolism, catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate. 5-methyltetrahydrofolate donates its methyl group for remethylation of homocysteine to methionine. Methionine is further converted to S-adenosylmethionine (SAM), a major reactive methyl carrier. DNA methyltransferases and histone methyltransferases use SAM to methylate DNA and histones with concomitant conversion of SAM to S-adenosylhomocysteine (SAH) (1, 2). In addition, MTHFR is inhibited by SAM and this feedback inhibition is partially reduced by SAH (3). Metabolically regulated levels of SAM and SAM/SAH ratio are shown to predict histone methylation levels, indicating the important role of enzymes in one-carbon metabolism including MTHFR in determining histone methylation status (4). |
| 存放说明 | -20C |
| 计算分子量 | 78 |
| 参考文献 | 1 . Ducker, G.S. and Rabinowitz, J.D. (2017) Cell Metab 25, 27-42. 2 . Feil, R. and Fraga, M.F. (2012) Nat Rev Genet 13, 97-109. 3 . Kutzbach, C. and Stokstad, E.L. (1971) Biochim Biophys Acta 250, 459-77. 4 . Mentch, S.J. et al. (2015) Cell Metab 22, 861-73. |
Western blot analysis of extracts from Daudi, Raji, and MOLT-4 cells using MTHFR (D1E4V) Rabbit mAb. |
