| 货号 | 49360S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , ChIP , ChIP-seq |
| 目标/特异性 | Brg1 (D1Q7F) Rabbit mAb recognizes endogenous levels of total Brg1 protein. This antibody does not cross-react with BRM protein. |
| 使用方法 | W IP ChIP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines (10-13). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84. 2 . Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73. 3 . Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11. 4 . Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81. 5 . Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17. 6 . Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32. 7 . Morettini, S. et al. (2008) Front Biosci 13, 5522-32. 8 . Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6. 9 . Simone, C. (2006) J Cell Physiol 207, 309-14. 10 . Yamamichi, N. et al. (2005) Oncogene 24, 5471-81. 11 . Reisman, D.N. et al. (2002) Oncogene 21, 1196-207. 12 . Shen, H. et al. (2008) Cancer Res 68, 10154-62. 13 . Weissman, B. and Knudsen, K.E. (2009) Cancer Res 69, 8223-30. |
Immunoprecipitation of Brg1 from NCCIT cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Brg1 (D1Q7F) Rabbit mAb. Western blot analysis was performed using Brg1 (D1Q7F) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Brg1 (D1Q7F) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min) and either 10 μl of Brg1 (D1Q7F) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human ESR1 Promoter Primers #9673, SimpleChIP® Human pS2 Promoter Primers #9702, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using Brg1 (D1Q7F) Rabbit mAb (upper) or BRM (D9E8B) XP®Rabbit mAb (lower). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d followed by treatment with β-estradiol (10 nM, 45 min) and 10 μl of Brg1 (D1Q7F) XP® Rabbit mAb, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. DNA Libraries were prepared from 5ng enriched ChIP DNA using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, and sequenced on the Illumina NextSeq. The figure shows binding across the ENSA gene. For additional ChIP-seq tracks, please download the product data sheet. |
