| 货号 | 49468S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Visinin-like Protein 1 (D9L6L) Rabbit mAb recognizes endogenous levels of total Visinin-like Protein 1 protein. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Visinin-like Protein 1, also called VILIP-1 and VSNL1, is a calcium-sensing protein in the central nervous system. Visinin-like Protein 1 exhibits a widespread distribution with high expression in the CNS, and lower expression levels in some peripheral tissues (1). Visinin-like Protein 1 responds to increased intracellular calcium concentration by translocating from the cytoplasm to membranes through calcium-dependent myristoylation at its N-terminus that allows interaction with membranes (2). This change in localization has been proposed to facilitate the activation of compartment-specific signal transduction for the selective activation of downstream signaling cascades (3,4). In Alzheimer’s disease, Visinin-like Protein 1 expression is decreased in the brain, including a reduced number of Visinin-like Protein 1 immunoreactive neurons (5); however, the presence of the protein is increased in the CSF (6), suggesting that it is released from neurons during insults. |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . Gierke, P. et al. (2004) Biochem Biophys Res Commun 323, 38-43. 2 . Kobayashi, M. et al. (1993) J Biol Chem 268, 18898-904. 3 . Spilker, C. et al. (2002) J Neurosci 22, 7331-9. 4 . Braunewell, K.H. et al. (1997) J Neurochem 68, 2129-39. 5 . Bernstein, H.G. et al. (1999) J Neurocytol 28, 655-62. 6 . Lee, J.M. et al. (2008) Clin Chem 54, 1617-23. |
Western blot analysis of extracts from various cell lines and tissues using Visinin-like Protein 1 (D9L6L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of Visinin-like Protein 1 from Huh7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 Visinin-like Protein 1 (D9L6L) Rabbit mAb. Western blot analysis was performed using Visinin-like Protein 1 (D9L6L) Rabbit mAb. |
