| 货号 | 44006S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Rat, Monkey |
| 应用 | WB,IP |
| 目标/特异性 | MSL2 (D4V2N) Rabbit mAb recognizes endogenous levels of total MSL2 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Male-specific lethal 2-homolog (MSL2) is a component of the male-specific lethal (MSL) histone acetyltransferase complex, which contains MSL1, MSL2, MSL3, and MYST1. MYST1, also known as mammalian male absent on the first (MOF) and lysine acetyltransferase 8 (KAT8), is a member of the MYST (MOZ, YBF2, SAS2, and Tip60) family of histone acetyltransferases (1,2) and functions as the catalytic subunit of the MSL complex. The MSL complex is responsible for the majority of acetylation on histone H4 lysine 16 (3-5). In addition, as part of the MSL complex, MSL1 and MSL2 function as an E3 ubiquitin ligase to mono-ubiquitylate histone H2B on lysine 34 (6). The MSL complex plays a critical role in the regulation of transcription, DNA repair, autophagy, apoptosis, and embryonic stem cell pluripotency and differentiation (1,2,6,7). Loss of MSL activity leads to a global reduction in histone H4 lysine 16 acetylation, a common hallmark found in many human cancers. In particular, the reduction of MYST1 protein levels and histone H4 lysine 16 acetylation is associated with poor prognosis in breast, renal, colorectal, gastric, and ovarian cancers (1). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Yang, Y. et al. (2014) Front Med 8, 79-83. 2 . Li, X. and Dou, Y. (2010) Epigenetics 5, 185-8. 3 . Dou, Y. et al. (2005) Cell 121, 873-85. 4 . Li, X. et al. (2009) Mol Cell 36, 290-301. 5 . Cai, Y. et al. (2010) J Biol Chem 285, 4268-72. 6 . Wu, L. et al. (2011) Mol Cell 43, 132-44. 7 . Füllgrabe, J. et al. (2013) Nature 500, 468-71. |
Western blot analysis of extracts from various cell lines using MSL2 (D4V2N) Rabbit mAb. |
