| 货号 | 53600S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | SHOC2 (D7N1A) Rabbit mAb recognizes endogenous levels of total SHOC2 protein. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SHOC2 is a scaffolding protein that harbors multiple leucine-rich repeats in tandem and is an upstream positive regulator of growth factor-dependent MAPK/ERK signaling. Research studies have demonstrated that SHOC2 forms a complex with the catalytic subunit of the PP1 phosphatase and M-Ras, and this complex drives activation of Raf-ERK signaling in response to mitogenic growth factors (1). SHOC2 has also been shown to cross-talk with and activate the PI3K/Akt signaling axis through its interaction with the p110α catalytic subunit of PI3K (2). As a positive regulator of ERK and PI3K/Akt signaling cascades, SHOC2 has been implicated in the regulation of several oncogenic cellular processes such as cell motility, invasion, and metastasis (2). A mutation in SHOC2that introduces an N-terminal myristoylation site, promotes aberrant membrane targeting of SHOC2, hyperactive MAPK/ERK signaling, and Noonan-like syndrome (3). |
| 存放说明 | -20C |
| 计算分子量 | 64 |
| 参考文献 | 1 . Rodriguez-Viciana, P. et al. (2006) Mol Cell 22, 217-30. 2 . Kaduwal, S. et al. (2015) Oncotarget 6, 33091-105. 3 . Cordeddu, V. et al. (2009) Nat Genet 41, 1022-6. |
Western blot analysis of extracts from various cell lines using SHOC2 (D7N1A) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SHOC2 protein (hSHOC2-Myc/DDK; +), using SHOC2 (D7N1A) Rabbit mAb. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human SHOC2 (+), using SHOC2 (D7N1A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). |
