| 货号 | 54551S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , F , IF-IC |
| 目标/特异性 | NKX6.1 (D8O4R) Rabbit mAb recognizes endogenous levels of total NKX6.1 protein. Note: Some non-specific staining of squamous epithelium has been observed by IHC. It is unclear if the antibody works on human samples in western blotting but is predicted to based on 100% sequence homology. |
| 使用方法 | W IP IHC-P IF-IC F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The homeodomain protein NKX6.1 is a transcription factor that regulates pancreatic β-cell development (1). Overexpressed NKX6.1 stimulates rat pancreatic β-cell proliferation and increases glucose-stimulated insulin secretion (GSIS) (2). The effect on GSIS was shown to be mediated by the up-regulation of prohormone VGF expression and the subsequent potentiation by TLQP-21, a peptide derived from VGF (3). Both nuclear receptors Nr4a1 and Nr4a3 are essential for pancreatic β-cell proliferation driven by overexpressed NKX6.1 (4). In addition, studies suggest that NKX6.1 is a suppressor for epithelial-to-mesenchymal transition (EMT), leading to inhibition of tumor metastasis (5). |
| 存放说明 | -20C |
| 计算分子量 | 44, 46 |
| 参考文献 | 1 . Habener, J.F. et al. (2005) Endocrinology 146, 1025-34. 2 . Schisler, J.C. et al. (2008) Mol Cell Biol 28, 3465-76. 3 . Stephens, S.B. et al. (2012) Cell Metab 16, 33-43. 4 . Tessem, J.S. et al. (2014) Proc Natl Acad Sci U S A 111, 5242-7. 5 . Li, H.J. et al. (2015) Oncogene , . |
Flow cytometric analysis of mIMCD-3 (blue) and β-TC-6 (green) cells using NKX6.1 (D8O4R) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of β-TC-6 (positive; left) or mIMCD-3 (negative; right) cells, using NKX6.1 (D8O4R) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using NKX6.1 (D8O4R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunohistochemical analysis of paraffin-embedded β-TC-6 (left) and mIMCD3 (right) cell pellets using NKX6.1 (D8O4R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human pancreas using NKX6.1 (D8O4R) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse pancreas using NKX6.1 (D8O4R) Rabbit mAb. |
