| 货号 | 46249S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human, Mouse, Monkey |
| 应用 | WB,IP,IHC,IF |
| 目标/特异性 | Exportin-1/CRM1 (D6V7N) Rabbit mAb recognizes endogenous levels of total exportin-1/CRM1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:50) Immunohistochemistry (Paraffin) (1:600) Immunofluorescence (Immunocytochemistry) (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Exportins are a family of seven proteins that are responsible for intracellular transport. Exportin-1, also known as chromosome region maintenance 1 (CRM1), is a protein essential for nuclear export of hundreds of proteins, mRNAs, and rRNAs (1-3). Exportin-1 binds to substrates with nuclear export signals (NESs) rich in leucine and other hydrophobic amino acids (4). These hydrophobic sequences form an alpha-helix-loop that can bind to the exportin-1 hydrophobic groove (5). Studies have shown that these NESs can be modified either by protein modifications or by mutation to regulate exportin-1 binding (6-7). Targets of exportin-1 include many tumor suppressors, such as Rb, p53, FoxO1, BAF47, as well as oncoproteins, such as p21 and p27 (1). In addition, Myc can upregulate exportin-1 during biogenesis, where it can export newly formed 40S and 60S subunits from the nucleoli (8-9). Inhibition of nuclear export has been pursued for therapeutic application since the finding that leptomycin B could suppress HIV replication by suppressing the ability of exportin-1 to export the HIV-1 protein Rev (2, 10). Overexpression of exportin-1 has been associated with poor prognosis in various cancer types (11-13). Genomic approaches and development of inhibitors have identified exportin-1 as a druggable target (14-16). The use of various inhibitors of exportin-1 is also being explored in various antiviral therapies (17-18). |
| 存放说明 | -20C |
| 计算分子量 | 123 |
| 参考文献 | 1 . Xu, D. et al. (2012) Mol Biol Cell 23, 3673-6. 2 . Ossareh-Nazari, B. et al. (1997) Science 278, 141-4. 3 . Fukuda, M. et al. (1997) Nature 390, 308-11. 4 . Kutay, U. and Güttinger, S. (2005) Trends Cell Biol 15, 121-4. 5 . Dong, X. et al. (2009) Nat Struct Mol Biol 16, 558-60. 6 . Vogt, P.K. et al. (2005) Cell Cycle 4, 908-13. 7 . Craig, E. et al. (2002) EMBO J 21, 31-42. 8 . Thomas, F. and Kutay, U. (2003) J Cell Sci 116, 2409-19. 9 . Golomb, L. et al. (2012) Mol Cell 45, 222-32. 10 . Wolff, B. et al. (1997) Chem Biol 4, 139-47. 11 . Noske, A. et al. (2008) Cancer 112, 1733-43. 12 . Shen, A. et al. (2009) Neurosurgery 65, 153-9; discussion 159-60. 13 . Yao, Y. et al. (2009) Oncol Rep 21, 229-35. 14 . Schmidt, J. et al. (2013) Leukemia 27, 2357-65. 15 . Boyle, S.M. et al. (1999) J Virol 73, 6872-81. 16 . Walker, C.J. et al. (2013) Blood 122, 3034-44. 17 . Yadav, V. et al. (2016) Virusdisease 27, 357-368. 18 . Chutiwitoonchai, N. et al. (2017) Virology 507, 32-39. |
Western blot analysis of extracts from various cell lines using Exportin-1/CRM (D6V7N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Exportin-1/CRM1 (D6V7N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human non-Hodgkins lymphoma using Exportin-1/CRM1 (D6V7N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Exportin-1/CRM1 (D6V7N) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Exportin-1/CRM1 (D6V7N) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Exportin-1/CRM1 (D6V7N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). |
