| 货号 | 62262S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IF-IC |
| 目标/特异性 | NQO1 (D6H3A) Rabbit mAb recognizes endogenous levels of total NQO1 protein. |
| 使用方法 | W IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQO1 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow (2). |
| 存放说明 | -20C |
| 计算分子量 | 29 |
| 参考文献 | 1 . Belinsky, M. and Jaiswal, A.K. (1993) Cancer Metastasis Rev. 12, 103-117. 2 . Siegel, D. et al. (1998) Clin. Cancer Res. 4, 2065-2070. 3 . Long, D.J. et al. (2002) Cancer Res. 62, 3030-3036. |
Confocal immunofluorescent analysis of DU 145 (positive, left) or Saos-2 (negative, right) cells using NQO1 (D6H3A) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using NQO1 (D6H3A) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). |
