| 货号 | 64407S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | GPR37 (D4C8H) Rabbit mAb recognizes endogenous levels of total GPR37 protein. This product does not cross-react with GPR37L1. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | GPR37 is a G protein-coupled receptor (GPCR) that was originally identified as an orphan receptor highly expressed in the brain and testis (1). It shares significant homology with the receptors of endothelin and bombesin peptides (1). Neuropeptide head activator from the invertebrate Hydra was identified as a high-affinity ligand of GPR37 (2), however, to date, no mammalian ortholog of this peptide that could represent an endogenous GPR37 ligand has been identified. Recently, GPR37 was deorphanized as the receptor for the endogenous peptides prosaptide and prosaposin (3). GPR37 is a substrate of the E3 ubiquitin ligase parkin, and is often referred to as “parkin-associated endothelin-like receptor,” or “Pael-R” (4). GPR37 has been implicated in the pathogenesis of Parkinson’s Disease as it aggregates in the substantia nigra of some PD patients (4,5). Interestingly, prosaposin exerts neuroprotective, neurotrophic, and gliotrophic actions (6), and GPR37 was identified as a negative regulator of oligodendrocyte differentiation and myelination (7), suggesting that it could represent a potential target for demyelinating pathologies. |
| 存放说明 | -20C |
| 计算分子量 | 40-250 |
| 参考文献 | 1 . Marazziti, D. et al. (1998) Genomics 53, 315-24. 2 . Rezgaoui, M. et al. (2006) J Cell Sci 119, 542-9. 3 . Meyer, R.C. et al. (2013) Proc Natl Acad Sci U S A 110, 9529-34. 4 . Imai, Y. et al. (2001) Cell 105, 891-902. 5 . Murakami, T. et al. (2004) Ann Neurol 55, 439-42. 6 . Misasi, R. et al. (2009) Cent Nerv Syst Agents Med Chem 9, 119-31. 7 . Yang, H.J. et al. (2016) Nat Commun 7, 10884. |
Western blot analysis of extracts from the indicated tissues using GPR37 (D4C8H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of GPR37 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is GPR37 (D4C8H) Rabbit mAb. Western blot analysis was performed using GPR37 (D4C8H) Rabbit mAb. |
