| 货号 | 65880S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | ATF-6 (D4Z8V) Rabbit mAb recognizes endogenous levels of total ATF-6 protein. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Activating Transcription Factor-6 (ATF-6) is a transmembrane glycoprotein of the endoplasmic reticulum (ER). Upon accumulation of improperly folded proteins in the ER, ATF-6 is cleaved liberating a 50 kDa amino-terminal fragment that translocates to the nucleus (1, 2). There, ATF-6 activates transcription of ER chaperones and components of ER-associated degradation such as GRP78, XBP-1, and CHOP (3-5). |
| 存放说明 | -20C |
| 计算分子量 | 90-100 |
| 参考文献 | 1 . Ye, J. et al. (2000) Mol Cell 6, 1355-64. 2 . Liu, C.Y. and Kaufman, R.J. (2003) J Cell Sci 116, 1861-2. 3 . Thuerauf, D.J. et al. (2007) J Biol Chem 282, 22865-78. 4 . Hirota, M. et al. (2006) J Toxicol Sci 31, 149-56. 5 . Todd, D.J. et al. (2008) Nat Rev Immunol 8, 663-74. |
Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin (1 mM; 1hr; +), using ATF-6 (D4Z8V) Rabbit mAb. | |
Immunoprecipitation of ATF-6 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ATF-6 (D4Z8V) Rabbit mAb. Western blot analysis was performed using ATF-6 (D4Z8V) Rabbit mAb. |
