| 货号 | 67774S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | Ch-TOG (D2Z8J) Rabbit mAb recognizes endogenous levels of total ch-TOG protein. Based on the amino acid sequence of the peptide antigen, this antibody is expected to recognize all isoforms of ch-TOG. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ch-TOG (colonic hepatic tumor overexpressed gene)/CKAP5 (cytoskeleton-associated protein 5) is a microtubule stabilizing protein involved in the organization of mitotic spindle poles through interaction with the transforming acid coiled-coil protein, TACC3 (1). Ch-TOG and TACC3 also interact with the membrane trafficking protein clathrin, and this interaction is thought to be required for clathrin’s mitotic function in crosslinking microtubules in the mitotic spindle (2). Researchers have found that expression levels of both TACC3 and ch-TOG are correlated with human diseases such as glioblastoma and hepatic carcinoma (3). A genome-wide siRNA screen identified ch-TOG and other G2/M phase regulators as potential contributors to head and neck squamous cell carcinoma (4). |
| 存放说明 | -20C |
| 计算分子量 | 225 |
| 参考文献 | 1 . Gergely, F. et al. (2003) Genes Dev 17, 336-41. 2 . Royle, S.J. (2012) J Cell Sci 125, 19-28. 3 . Thakur, H.C. et al. (2013) Biol Chem 394, 1411-23. 4 . Martens-de Kemp, S.R. et al. (2013) Clin Cancer Res 19, 1994-2003. |
Western blot analysis of extracts from MCF7 cells, transfected with control siRNA (-) or ch-TOG siRNA (+), using Ch-TOG (D2Z8J) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The Ch-TOG (D2Z8J) Rabbit mAb confirms silencing of ch-TOG expression, while the α-Actinin (D6F6) XP® Rabbit mAb is used as a loading control. | |
Western blot analysis of extracts from various cell lines using Ch-TOG (D2Z8J) Rabbit mAb. |
