| 货号 | 72908S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB |
| 目标/特异性 | PEAK1 (D4G6J) Rabbit mAb recognizes endogenous levels of total PEAK1 protein. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PEAK1 (Pseudopodium-enriched atypical kinase 1 or sgk269) is a member of nonreceptor atypical tyrosine kinase family identified by MS analysis of purified psedopodium (1). PEAK1 is a multi-domain protein with a N-terminal Erk binding site, followed by actin-targeting/Src substrate/Erk substrate region, Crk binding site, Shc binding site, and a C-terminal kinase domain (1, 2). By interacting with different adaptors like Shc, Grb2, Src, and others, PEAK1 functions as an important regulator in different signaling pathways, namely the Src/PEAK1/ErbB2 (3), EGFR Shc1/PEAK1/Grb2(4), TGFβ/PEAK1/Src/MAPK (5), and fibronectin/PEAK1/Src (6) pathways. PEAK1 plays an instrumental role in a wide variety of biological processes including epithelial-mesenchymal transition (EMT), dynamics of focal adhesion, cancer metastatic growth and invasion as well as cancer drug resistance (3, 5-8). Phosphorylation of PEAK1 at Tyr665 or Tyr635 by SFK (Src family Kinases) has been shown to be essential for cancer cell migration and invasion as well as the turnover of focal adhesions (7, 9). |
| 存放说明 | -20C |
| 计算分子量 | 250 |
| 参考文献 | 1 . Wang, Y. et al. (2010) Proc Natl Acad Sci U S A 107, 10920-5. 2 . Kelber, J.A. and Klemke, R.L. (2010) Oncotarget 1, 219-23. 3 . Kelber, J.A. et al. (2012) Cancer Res 72, 2554-64. 4 . Zheng, Y. et al. (2013) Nature 499, 166-71. 5 . Agajanian, M. et al. (2015) PLoS One 10, e0135748. 6 . Agajanian, M. et al. (2015) Biochem Biophys Res Commun 465, 606-12. 7 . Bristow, J.M. et al. (2013) J Biol Chem 288, 123-31. 8 . Fujimura, K. et al. (2014) Cancer Res 74, 6671-81. 9 . Croucher, D.R. et al. (2013) Cancer Res 73, 1969-80. |
Western blot analysis of extracts from H226, SK-MEL-28, and HEK-293 cell lines using PEAK1 (D4G6J) Rabbit mAb. |
