| 货号 | 77934S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | TRPC3 (D4P5S) Rabbit mAb recognizes endogeneous levels of total TRPC3 protein. This antibody does not cross-react with TRPC6 or TRPC7 proteins. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Transient Receptor Potential Canonical 3 (TRPC3) belongs to the superfamily of TRP cation channels. The TRPC subfamily (TRPC1-7) is a group of calcium-permeable cation channels that mediates the increase in intracellular [Ca2+] following activation by G-protein-coupled receptors or receptor tyrosine kinases (1). TRPC3 is mainly expressed in the brain and various other tissues, though at a much lower level (2). In the brain, TRPC3 is involved in BDNF-induced axonal growth cone plasticity, dendritic spine formation, and neuronal survival (3-6). It is also required for synaptic transmission and motor coordination (7). Outside of the central nervous system, TRPC3 also exerts other important biological functions such as regulating cardiac and vascular contractility, maintaining Ca2+ homeostasis in primary T cells and endothelial cells (8-10). TRPC3 is activated by diacylglycerol and Inositol 1,4,5-trisphosphate (11,12). It is also activated by internal calcium store depletion and regulates mitochondrial calcium uptake (13,14). |
| 存放说明 | -20C |
| 计算分子量 | 105 |
| 参考文献 | 1 . Montell, C. (2005) Sci STKE 2005, re3. 2 . Riccio, A. et al. (2002) Brain Res Mol Brain Res 109, 95-104. 3 . Li, H.S. et al. (1999) Neuron 24, 261-73. 4 . Li, Y. et al. (2005) Nature 434, 894-8. 5 . Amaral, M.D. and Pozzo-Miller, L. (2007) J Neurosci 27, 5179-89. 6 . Jia, Y. et al. (2007) Nat Neurosci 10, 559-67. 7 . Hartmann, J. et al. (2008) Neuron 59, 392-8. 8 . Nilius, B. et al. (2003) Endothelium 10, 5-15. 9 . Yeon, S.I. et al. (2014) PLoS One 9, e110413. 10 . Wenning, A.S. et al. (2011) Biochim Biophys Acta 1813, 412-23. 11 . Hofmann, T. et al. (1999) Nature 397, 259-63. 12 . Song, T. et al. (2015) Am J Physiol Lung Cell Mol Physiol 309, L1455-66. 13 . Ma, H.T. et al. (2000) Science 287, 1647-51. 14 . Feng, S. et al. (2013) Proc Natl Acad Sci U S A 110, 11011-6. |
Western blot analysis of extracts from human, mouse, and rat brain using TRPC3 (D4P5S) Rabbit mAb. | |
Immunoprecipitation of TRPC3 from Rat brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is TRPC3 (D4P5S) Rabbit mAb. Western blot analysis was performed using |
