| 货号 | 81694S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , F , IF-IC |
| 目标/特异性 | Brachyury (D2Z3J) Rabbit mAb recognizes endogenous levels of total Brachyury protein. |
| 使用方法 | W IP IF-IC F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Brachyury protein, encoded by the T gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis (1). In the mouse, brachyury is necessary for mesodermal morphogenetic cell movements during gastrulation. Brachyury mutant mice die in utero and display deficient mesoderm formation including an abnormal notochord, missing posterior somites, and a reduced allantois (2). Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors (3,4). A common polymorphism in the human T gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Edwards, Y.H. et al. (1996) Genome Res 6, 226-33. 2 . Wilson, V. et al. (1995) Development 121, 877-86. 3 . Vujovic, S. et al. (2006) J Pathol 209, 157-65. 4 . Yang, X.R. et al. (2009) Nat Genet 41, 1176-8. 5 . Morrison, K. et al. (1996) Hum Mol Genet 5, 669-74. 6 . Jensen, L.E. et al. (2004) Hum Genet 115, 475-82. |
Confocal immunofluorescent analysis of MUG-CHOR-1 (positive, left) and MCF 10A (negative, right) cells using Brachyury (D2Z3J) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). | |
Flow cytometric analysis of MCF 10A cells (blue) and MUG-CHOR-1 cells (green) using Brachyury (D2Z3J) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from NCI-H460, MUG-CHOR-1, and UCH2 cell lines using Brachyury (D2Z3J) Rabbit mAb. |
