| 货号 | 83029S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IHC-P , IF-IC |
| 目标/特异性 | EphB2 (D2X2I) Rabbit mAb recognizes endogenous levels of total EphB2 protein. |
| 使用方法 | W IP IHC-P IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The ephrin receptor B2 (EphB2) is an ephrin family receptor tyrosine kinase that plays an important role in regulating growth and development of multiple tissues and organs (1,2). The EphB2 transmembrane receptor protein contains a kinase domain, a PDZ motif, and a SAM domain within a conserved cytoplasmic domain. A ligand binding domain, a cysteine-rich domain, and fibronectin type III repeats comprise the conserved EphB2 extracellular domain (3). EphB2 binds with high affinity to ephrin B ligands, and to some ephrin A proteins, to initiate bidirectional signaling between neighboring cells (1,2). Upon binding, EphB2-Ephrin B2 dimers form a heterotetramer and position the receptor-ligand complex on the cell membrane to facilitate bidirectional signal transduction (3). In addition to associating with ephrin ligands, EphB2 also regulates a number of biological processes through interaction with focal adhesion kinase (FAK), NMDA receptor (NMDAR), the Rac1 guanine nucleotide exchange factor Tiam1, and p21-activated kinase (PAK1) (4-7). While some studies support a role for EphB2 as a pro-oncogenic kinase, other research suggests that EphB2 acts as a tumor suppressor (1,2,4,8). |
| 存放说明 | -20C |
| 计算分子量 | 130 |
| 参考文献 | 1 . Pasquale, E.B. (2008) Cell 133, 38-52. 2 . Klein, R. (2009) Nat Neurosci 12, 15-20. 3 . Himanen, J.P. et al. (2001) Nature 414, 933-8. 4 . Wang, S.D. et al. (2012) Oncogene 31, 5132-43. 5 . Nolt, M.J. et al. (2011) J Neurosci 31, 5353-64. 6 . Tolias, K.F. et al. (2007) Proc Natl Acad Sci U S A 104, 7265-70. 7 . Srivastava, N. et al. (2013) Mol Cell Neurosci 52, 106-16. 8 . Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65. |
Confocal immunofluorescent analysis of COLO 205 (positive; left) and K-562 (negative; right) cells using EphB2 (D2X2I) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using EphB2 (D2X2I) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using EphB2 (D2X2I) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using EphB2 (D2X2I) Rabbit mAb. | |
Immunoprecipitation of EphB2 protein from Saos-2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is EphB2 (D2X2I) Rabbit mAb. Western blot analysis was performed using EphB2 (D2X2I) Rabbit mAb. |
