| 货号 | 83609S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | Claudin-3 (D7A3O) Rabbit mAb recognizes endogenous levels of total claudin-3 protein. |
| 使用方法 | W IP IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Tight junctions, or zonula occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 20 |
| 参考文献 | 1 . Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. 2 . Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28. 3 . Hewitt, K.J. et al. (2006) BMC Cancer 6, 186. 4 . Brandner, J.M. et al. (2002) Eur J Cell Biol 81, 253-63. 5 . Krämer, F. et al. (2000) Hum Genet 107, 249-56. 6 . Swisshelm, K. et al. (1999) Gene 226, 285-95. 7 . Choi, Y.L. et al. (2007) Histol Histopathol 22, 1185-95. 8 . Hudson, L.G. et al. (2008) Clin Exp Metastasis 25, 643-55. 9 . Todd, M.C. et al. (2015) Oncol Lett 10, 156-162. 10 . Herschkowitz, J.I. et al. (2012) Proc Natl Acad Sci U S A 109, 2778-83. |
Western blot analysis of extracts from various cell lines using Claudin-3 (D7A3O) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Confocal immunofluorescent analysis of T-47D (left) and Saos-2 (right) cells using Claudin-3 (D7A3O) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Immunoprecipitation of claudin-3 protein from T-47D cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Claudin-3 (D7A3O) Rabbit mAb. Western blot analysis was performed using Claudin-3 (D7A3O) Rabbit mAb. |
