| 货号 | 84546S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | CXXC5 (D1O4P) Rabbit mAb recognizes endogenous levels of total CXXC5 protein. |
| 使用方法 | W IP IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The CXXC-type zinc finger protein 5 (CXXC5) is a nuclear protein that regulates gene expression and is involved in the regulation of cell growth and differentiation, apoptosis, cell adhesion, and cytoskeletal organization. The CXXC5 protein is also known as retinoid-inducible nuclear factor (RINF) as it was originally identified from a set of genes upregulated by retinoic acid stimulation (1). CXXC5 is a transcriptional activator of the vascular endothelial growth factor receptor VEGFR2. The CXXC5 protein regulates differentiation and migration of endothelial cells and subsequent blood vessel formation downstream of bone morphogenic protein (BMP) signaling (2). CXXC5 also regulates TNFα-induced apoptosis by facilitating phosphorylation of Smad3 and the nuclear translocation of Smad4 (3). Expression of CXXC5 in skeletal muscle regulates expression of genes involved in skeletal myogenesis (4). This nuclear factor also plays an important role in the regulation of normal myelopoiesis. The CXXC5 gene is localized to the 5q31.2 chromosomal region that is often involved in abnormalities associated with various myeloid malignancies, and CXXC5 over-expression is associated with decreased overall survival in human AML (5). Interestingly, CXXC5 is also over-expressed in many solid tumors, and high expression is also correlated with poor prognosis in breast cancer (6). |
| 存放说明 | -20C |
| 计算分子量 | 33 |
| 参考文献 | 1 . Pendino, F. et al. (2009) Blood 113, 3172-81. 2 . Kim, H.Y. et al. (2014) FASEB J 28, 615-26. 3 . Wang, X. et al. (2013) Curr Mol Med 13, 1385-96. 4 . Li, G. et al. (2014) J Muscle Res Cell Motil 35, 259-65. 5 . Astori, A. et al. (2013) Oncotarget 4, 1438-48. 6 . Knappskog, S. et al. (2011) Ann Oncol 22, 2208-15. |
Confocal immunofluorescent analysis of T-47D (left) and HT-1080 (right) cells using CXXC5 (D1O4P) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). As expected, CXXC5 expression is low in HT-1080 cells. | |
Western blot analysis of extracts from various cell lines using CXXC5 (D1O4P) Rabbit mAb. | |
Immunoprecipitation of CXXC5 from T-47D extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CXXC5 (D1O4P) Rabbit mAb. Western blot analysis was performed using CXXC5 (D1O4P) Rabbit mAb. |
