| 货号 | 85325S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | PICK1 (D6W7M) Rabbit mAb recognizes endogenous levels of total PICK1 protein. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PICK1, or Protein interacting with C-kinase 1, is a cytosolic adaptor protein composed of an N-terminus PDZ domain and a C-terminus BAR domain that allow protein and membrane interactions, respectively (1,2). PICK1 regulates endosomal trafficking and surface expression of AMPA receptors and is therefore involved in synaptic plasticity (3). PICK1 is a negative regulator of Arp2/3-dependent actin polymerization and also for the development of neuronal architecture (4). Finally, increasing evidence indicates that PICK1 expression is upregulated in a number cancers (5), and that PICK1 interacts with proteins involved in the promotion of tumorigenesis such as Ephrin receptors, Coxsackie-adenovirus receptor (CAR), and ErbB2/Her2 (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Staudinger, J. et al. (1995) J Cell Biol 128, 263-71. 2 . Jin, W. et al. (2006) J Neurosci 26, 2380-90. 3 . Xia, J. et al. (1999) Neuron 22, 179-87. 4 . Rocca, D.L. et al. (2008) Nat Cell Biol 10, 259-71. 5 . Zhang, B. et al. (2010) Cancer Sci 101, 1536-42. 6 . Jaulin-Bastard, F. et al. (2001) J Biol Chem 276, 15256-63. 7 . Excoffon, K.J. et al. (2004) J Cell Sci 117, 4401-9. |
Immunoprecipitation of PICK1 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PICK1 (D6W7M) Rabbit mAb. Western blot analysis was performed using PICK1 (D6W7M) Rabbit mAb. | |
Western blot analysis of extracts from various tissues using PICK1 (D6W7M) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing MycDDK-tagged full-length human PICK1 protein (hPICK1-MycDDK; +), using PICK1 (D6W7M) Rabbit mAb. |
