| 货号 | 71655S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Mouse |
| 应用 | WB,IP,IHC,IF,F |
| 目标/特异性 | EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred) recognizes endogenous levels of total mouse EGF receptor protein. This antibody cross-reacts very weakly with overexpressed human EGF receptor. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) Immunohistochemistry (Paraffin) (1:100) Immunofluorescence (Frozen) (1:400) Immunofluorescence (Immunocytochemistry) (1:400) Flow Cytometry (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10). |
| 存放说明 | -20C |
| 计算分子量 | 175 |
| 参考文献 | 1 . Hackel, P.O. et al. (1999) Curr Opin Cell Biol 11, 184-9. 2 . Zwick, E. et al. (1999) Trends Pharmacol Sci 20, 408-12. 3 . Cooper, J.A. and Howell, B. (1993) Cell 73, 1051-4. 4 . Hubbard, S.R. et al. (1994) Nature 372, 746-54. 5 . Biscardi, J.S. et al. (1999) J Biol Chem 274, 8335-43. 6 . Emlet, D.R. et al. (1997) J Biol Chem 272, 4079-86. 7 . Levkowitz, G. et al. (1999) Mol Cell 4, 1029-40. 8 . Ettenberg, S.A. et al. (1999) Oncogene 18, 1855-66. 9 . Rojas, M. et al. (1996) J Biol Chem 271, 27456-61. 10 . Feinmesser, R.L. et al. (1999) J Biol Chem 274, 16168-73. |
Confocal immunofluorescent analysis of mIMCD-3 cells (high expressing; left) and NIH/3T3 cells (low expressing; right) using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of mouse liver (high expressing; left) and mouse pancreas (low expressing; right) using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various mouse cell lines and tissues using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of EGF receptor protein from mIMCD-3 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred). Western blot analysis was performed using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred). | |
Immunohistochemical analysis of paraffin-embedded mouse forestomach using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred). | |
Immunohistochemical analysis of paraffin-embedded mouse kidney using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred). | |
Immunohistochemical analysis of paraffin-embedded mouse liver using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred). | |
Immunohistochemical analysis of paraffin-embedded mouse placenta using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred). | |
Flow cytometric analysis of NIH/3T3 cells (blue) and mIMCD-3 cells (green) using EGF Receptor (D1P9C) Rabbit mAb (Mouse Preferred). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. |
