| 货号 | 89670S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-IC |
| 目标/特异性 | HNF1α (D7Z2Q) Rabbit mAb recognizes endogenous levels of total HNF1α protein. This antibody does not cross-react with HNF1β protein. |
| 使用方法 | W IP IF-IC |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Hepatocyte nuclear factor 1α (HNF1α, also known as TCF1 or MODY3) is a transcription factor that plays a role in the tissue-specific regulation of liver gene expression (1). Research has shown that heterogeneous mutations of HNF1α are linked to maturity onset diabetes of the young (MODY) (2). Recent studies indicate that increased concentrations of free fatty acids can reduce the expression of FoxA2/HNF3β and HNF1α in pancreatic β-cells and lead to their nuclear exclusion, resulting in symptoms of several metabolic syndromes (3). |
| 存放说明 | -20C |
| 计算分子量 | 81 |
| 参考文献 | 1 . Yamagata, K. et al. (1996) Nature 384, 455-8. 2 . Lehto, M. et al. (1997) J Clin Invest 99, 582-91. 3 . Ohtsubo, K. et al. (2011) Nat Med 17, 1067-75. |
Confocal immunofluorescent analysis of Hep G2 (top left), HeLa (top right), H-4-II-E (bottom left), and KNRK (bottom right) cells using HNF1α (D7Z2Q) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). | |
Western blot analysis of extracts from various cell lines using HNF1α (D7Z2Q) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of HNF1α from INS-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HNF1α (D7Z2Q) Rabbit mAb. Western blot analysis was performed using HNF1α (D7Z2Q) Rabbit mAb. |
