| 货号 | 92356S |
| 同种亚型 | Mouse IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse IgG1 |
| 应用 | WB |
| 目标/特异性 | ATM (11G12) Mouse mAb recognizes endogenous levels of total ATM protein. |
| 使用方法 | W |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 350 |
| 参考文献 | 1 . Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8. 2 . Tang, X. et al. (2008) Mol Cell Biol 28, 2559-66. 3 . Shiloh, Y. (1997) Annu Rev Genet 31, 635-62. 4 . Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6. |
Western blot analysis of extract from HeLa, 293, and GM01588A (ATM-negative) cells using ATM (11G12) Mouse mAb (upper) or α-Actinin (D6F6) Rabbit mAb #6487 (lower). |
