| 货号 | 94063S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP |
| 目标/特异性 | Spred1 (D6D8B) Rabbit mAb recognizes endogenous levels of total Spred1 protein. |
| 使用方法 | W IP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Spred1 belongs to the Spred family functioning as sprouty-related suppressor of Ras signaling down stream of growth receptor signaling (1,2). The protein has a N-terminal EVH1 (Ena/VASP homology-1) domain, followed by a KBD (c-Kit- binding domain) and a C-terminal SPR (Sprouty) domain. The C-terminal SPR domain functions to translocate Spred1 to the membrane following growth factor stimulation (1,3). The N-terminal EVH1 domain interacts with neurofibromin to bring the later to the membrane proximal signaling complex for down regulation of Ras signaling (4,5). Mutations of Spred1 are the causes of Legius Syndrome (6,7). Spred1 expression inhibits tumor cell motility and metastasis, and down regulation has been found in hepatocarcinoma and myeloblastic leukemia (8-10). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Wakioka, T. et al. (2001) Nature 412, 647-51. 2 . Kato, R. et al. (2003) Biochem Biophys Res Commun 302, 767-72. 3 . King, J.A. et al. (2005) Biochem J 388, 445-54. 4 . Stowe, I.B. et al. (2012) Genes Dev 26, 1421-6. 5 . Hirata, Y. et al. (2015) J Biol Chem , . 6 . Brems, H. et al. (2007) Nat Genet 39, 1120-6. 7 . Brems, H. and Legius, E. (2013) Keio J Med 62, 107-12. 8 . Miyoshi, K. et al. (2004) Oncogene 23, 5567-76. 9 . Yoshida, T. et al. (2006) Oncogene 25, 6056-66. 10 . Pasmant, E. et al. (2015) Oncogene 34, 631-8. |
Western blot analysis of extracts from COLO 201, SK-MEL-5, and SK-MEL-2 cell lines using Spred1 (D6D8B) Rabbit mAb. | |
Immunoprecipitation of Spred1 protein from SK-MEL-5 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Spred1 (D6D8B) Rabbit mAb. Western blot analysis was performed using |
