| 货号 | 94725S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, IP , IF-F |
| 目标/特异性 | KCC2 (D1R2R) Rabbit mAb recognizes endogenous levels of total KCC2 protein. |
| 使用方法 | W IP IF-F |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The potassium/chloride cotransporter 2 (KCC2, SLC12A5) is a neuron-specific transport protein responsible for regulating the cotransport of potassium and chloride ions. KCC2 uses the energy of the electrochemical potassium gradient to export chloride ions from cells, therefore maintaining intracellular chloride ion concentrations in mature neurons (1,2). The intracellular concentration of chloride ions determines the neuronal response to the inhibitory neurotransmitter GABA and glycine. As a result, KCC2 can play a critical role in regulating neuronal excitability in mature central nervous system neurons (3-5). Altered KCC2 expression and reduced KCC2 activity can result in an increase in intracellular chloride ion concentrations and subsequent hyperexcitability of neuronal systems. Cases of aberrant KCC2 function are associated with neurological disorders, such as multiple forms of epilepsy, neuropathic pain, and schizophrenia (6-10). |
| 存放说明 | -20C |
| 计算分子量 | 130-260 |
| 参考文献 | 1 . Payne, J.A. et al. (1996) J Biol Chem 271, 16245-52. 2 . Delpire, E. (2000) News Physiol Sci 15, 309-12. 3 . Rivera, C. et al. (1999) Nature 397, 251-5. 4 . Woo, N.S. et al. (2002) Hippocampus 12, 258-68. 5 . Zhu, L. et al. (2005) J Neurophysiol 93, 1557-68. 6 . Arion, D. and Lewis, D.A. (2011) Arch Gen Psychiatry 68, 21-31. 7 . Hyde, T.M. et al. (2011) J Neurosci 31, 11088-95. 8 . Kaila, K. et al. (2014) Curr Opin Neurobiol 26, 34-41. 9 . Mòdol, L. et al. (2014) Pain 155, 1577-90. 10 . Kahle, K.T. et al. (2014) EMBO Rep 15, 766-74. |
Confocal immunofluorescent analysis of rat retina using KCC2 (D1R2R) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from human cerebellum, mouse and rat brain using KCC2 (D1R2R) Rabbit mAb. | |
Immunoprecipitation of KCC2 from mouse brain extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is KCC2 (D1R2R) Rabbit mAb. Western blot analysis was performed using KCC2 (D1R2R) Rabbit mAb. |
