| 货号 | 99169S |
| 同种亚型 | Rabbit IgG |
| 反应种属 | Human\Mouse\Rat\Moneky |
| 来源宿主 | Rabbit IgG |
| 应用 | WB, ChIP |
| 目标/特异性 | MBD3 (D1B8F) Rabbit mAb recognizes endogenous levels of total MBD3 protein. |
| 使用方法 | W ChIP |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Methyl-CpG-binding protein 2 (MeCP2) is the founding member of a family of methyl-CpG-binding domain (MBD) proteins that also includes MBD1, MBD2, MBD3, MBD4, MBD5 and MBD6 (1-3). Apart from MBD3, these proteins bind methylated cytosine residues in the context of the di-nucleotide 5´-CG-3´ to establish and maintain regions of transcriptionally inactive chromatin by recruiting a variety of co-repressor proteins (2). MeCP2 recruits histone deacetylases HDAC1 and HDAC2, and the DNA methyltransferase DNMT1 (4-6). MBD1 couples transcriptional silencing to DNA replication and interacts with the histone methyltransferases ESET and SUV39H1 (7,8). MBD2 and MBD3 co-purify as part of the NuRD (nucleosome remodeling and histone de-acetylation) co-repressor complex, which contains the chromatin remodeling ATPase Mi-2, HDAC1 and HDAC2 (9,10). MBD5 and MBD6 have recently been identified and little is known regarding their protein interactions. MBD proteins are associated with cancer and other diseases; MBD4 is best characterized for its role in DNA repair and MBD2 has been linked to intestinal cancer (11,12). Mutations in the MeCP2 gene cause the neurologic developmental disorder Rett Syndrome (13). MeCP2 protein levels are high in neurons, where it plays a critical role in multiple synaptic processes (14). In response to various physiological stimuli, MeCP2 is phosphorylated on Ser421 and regulates the expression of genes controlling dendritic patterning and spine morphogenesis (14). Disruption of this process in individuals with altered MeCP2 may cause the pathological changes seen in Rett Syndrome. |
| 存放说明 | -20C |
| 计算分子量 | 32, 34 |
| 参考文献 | 1 . Clouaire, T. and Stancheva, I. (2008) Cell Mol Life Sci 65, 1509-22. 2 . Hendrich, B. and Bird, A. (1998) Mol Cell Biol 18, 6538-47. 3 . Roloff, T.C. et al. (2003) BMC Genomics 4, 1. 4 . Nan, X. et al. (1998) Nature 393, 386-9. 5 . Jones, P.L. et al. (1998) Nat Genet 19, 187-91. 6 . Fuks, F. et al. (2003) J Biol Chem 278, 4035-40. 7 . Sarraf, S.A. and Stancheva, I. (2004) Mol Cell 15, 595-605. 8 . Fujita, N. et al. (2003) J Biol Chem 278, 24132-8. 9 . Zhang, Y. et al. (1999) Genes Dev 13, 1924-35. 10 . Wade, P.A. et al. (1999) Nat Genet 23, 62-6. 11 . Hendrich, B. et al. (1999) Nature 401, 301-4. 12 . Sansom, O.J. et al. (2003) Nat Genet 34, 145-7. 13 . Miltenberger-Miltenyi, G. and Laccone, F. (2003) Hum Mutat 22, 107-15. 14 . Zhou, Z. et al. (2006) Neuron 52, 255-69. |
Western blot analysis of extracts from various cell lines using MBD3 (D1B8F) Rabbit mAb. | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 K562 cells and either 10 µl of MBD3 (D1B8F) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, human BCR exon 1 primers, and SimpleChIP® Human Nanog Promoter Primers #95064. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
