货号 | 2372S |
来源宿主 | Mouse |
应用 | W/IP/IF-IC/F |
使用方法 | WB(1:1000) IP (1:50) F (1:7000) IF-IC (1:3200) |
供应商 | CST |
背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
存放说明 | -20C |
Western blot analysis of cells expressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb.Weatern blot分析表达β-半乳糖苷酶的细胞(泳道1),泳道2为未转染细胞,所用抗体为β-Gal (14B7) Mouse mAb。 | |
Immunoprecipitation of cell extracts overexpressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb, followed by Western blot analysis using the same antibody.免疫沉淀分析表达β-半乳糖苷酶的细胞(泳道1),泳道2为未转染组,所用抗体为β-Gal (14B7) Mouse mAb。然后用相同的抗体进行western blot 。 | |
Immunocytochemical staining of cells expressing b-galactosidase (left) or cells without transfection (right), using b-Gal 14B7 Mouse mAb.免疫细胞化学分析表达β-半乳糖苷酶的细胞(左图),右图为未转染细胞,所用抗体为β-Gal (14B7) Mouse mAb。 | |
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with β-galactosidase (green), using β-Gal (14B7) Mouse mAb.流式细胞术分析COS细胞,蓝色为未转染组,绿色为β-半乳糖苷酶转染组,所用抗体为β-Gal (14B7) Mouse mAb。 |