| 货号 | 4466S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-F |
| 目标/特异性 | β3-Tubulin (TU-20) Mouse mAb detects endogenous levels of total β3-tubulin protein and does not cross-react with tubulin isoforms expressed in non-neuronal cells. This clone is similar to TUJ1. |
| 使用方法 | WB(1:1000) IHC-P (1:100) IF-F (1:200) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from fetal rat and neonatal mouse brain using β3-Tubulin (TU-20) Mouse mAb. Western blot分析胎大鼠和新生小鼠大脑提取物，使用的抗体是β3-Tubulin (TU-20) Mouse mAb 鼠单抗。 | |
Confocal immunofluorescent image of rat cerebellum labeled with β3-Tubulin (TU-20) Mouse mAb (green) and Neurofilament-L (C28E10) Rabbit mAb #2837 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析大鼠小脑，使用的抗体是β3-Tubulin (TU-20)Mouse mAb 鼠单抗（绿）和Neurofilament-L (C28E10)Rabbit mAb 兔单抗#2837（红）。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded rat brain using beta3-Tubulin (TU-20) Mouse mAb. 免疫组化分析石蜡包埋的大鼠大脑切片，使用的抗体是β3-Tubulin (TU-20)Mouse mAb 鼠单抗。 |
