β2-Chimerin (2E3) Rat mAb
货号：
4728S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 4728S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rat IgG2a |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | Chimerins are a family of GTPase-activating proteins (GAPS) that facilitate GTP hydrolysis by the small GTPase Rac, rendering it inactive and regulating cell shape, spreading and motility. Regulation of chimerin proteins occurs in response to growth factor receptor or G-protein coupled receptor activation followed by phospholipase C activation. Chimerins are among the growing number of phorbol ester and diacylglycerol (DAG) effector molecules that do not belong to the PKC family of isoenzymes (reviewed in 1,2). β2-chimerin is highly expressed in brain and pancreas, and its expression is down-regulated in malignant gliomas (3). β2-chimerin is also down-regulated in breast cancer, and its expression causes GAP activity-dependent cell cycle arrest in MCF-7 breast cancer cells (4). Signaling from the epidermal growth factor receptor (EGFR) activates β2-chimerin and allows its association with Rac1 at the plasma membrane (5). Also in response to EGF, diacylglycerol kinase (DGK) γ interacts with β2-chimerin, promotes its translocation to the plasma membrane, and regulate its GAP activity (6).Chimerins 蛋白是GTP酶活化蛋白（GTPase activating proteins，GAPS)家族成员，该蛋白通过small GTPase Rac促进GTP水解，从而致使它的失活，并且调节细胞形态、传递和迁移。chimerin蛋白的调节出现在生长因子受体和G蛋白偶联受体激活及随后磷脂酶C的激活反应中。Chimerins 蛋白是越来越多的佛波酯和甘油二酯(DAG)的效应分子，但是不属于PKC同工酶家族（1,2）。β2-chimerin蛋白是在大脑和胰腺中高表达，在胶质瘤中它的表达下调(3)。β2-chimerin蛋白也在乳腺癌中下调表达，并且在 MCF-7乳腺癌细胞中它的表达引起 GAP活性依赖的细胞周期抑制(4)。表皮生长因子受体（epidermal growth factor receptor ，EGFR）信号激活β2-chimerin蛋白，并且允许该蛋白与细胞质膜的Rac1蛋白联系(5)。diacylglycerol kinase (DGK) γ 与 β2-chimerin蛋白相互作用，EGF作用下促进 β2-chimerin蛋白定位于细胞质膜，并且调节它的GAP活性(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 47 |
参考图片Western blot analysis of extracts from mouse brain, rat brain and SH-SY5Y cells using β2-Chimerin (2E3) Rat mAb.使用β2-Chimerin (2E3) Rat mAb，免疫印迹（Western Blot）分析小鼠大脑、大鼠大脑和SH-SY5Y细胞系中β2-Chimerin (2E3)蛋白水平。 |
