| 货号 | 3873S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC |
| 目标/特异性 | α-Tubulin (DM1A) Mouse mAb detects endogenous levels of total α-tubulin protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:4000) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
Western blot analysis of extracts from HeLa and COS-7 cells using α-Tubulin (DM1A) Mouse mAb. 使用α-Tubulin (DM1A) Mouse mAb，免疫印迹（Western Blot）分析HeLa和COS-7细胞中α-Tubulin蛋白水平。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using α-Tubulin (DM1A) Mouse mAb. 使用α-Tubulin (DM1A) Mouse mAb，免疫组化分析人源结肠癌组织石蜡切片。 | |
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb (green). Red = Propidium Iodide (fluorescent DNA dye). 使用α-Tubulin (DM1A) Mouse mAb (绿色)，共聚焦免疫荧光观察HeLa细胞。红色= Propidium Iodide (DNA荧光染料)。 |
