| 货号 | 2125S |
| 反应种属 | Human/Mouse/Rat/Monkey/Mink/D.melanogaster/Zebrafish/Bovine/Pig |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IF-IC/F |
| 目标/特异性 | α-Tubulin (11H10) Rabbit mAb detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin. |
| 使用方法 | WB(1:1000) IHC-P (1:50) F (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
Flow cytometric analysis of HeLa cells, using α-Tubulin (11H10) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). 与nonspecific negative control antibody (红色)比较，使用α-Tubulin (11H10) Rabbit mAb兔单抗 (蓝色)标记，流式细胞仪分HeLa细胞。 | |
Confocal immunofluorescent analysis of HeLa cells, using α-Tubulin (11H10) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
使用α-Tubulin (11H10) Rabbit mAb 兔单抗(绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。蓝色伪彩= DRAQ5® #4084 (DNA荧光染料)。 | |
