α-N-Catenin (C12G4) Rabbit mAb【科瑞奥博】

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α-N-Catenin (C12G4) Rabbit mAb

货号： 2163S 基本售价： 3360.0 元规格： -

产品信息

概述
货号	2163S
反应种属	Human/Mouse/Rat
来源宿主	Rabbit
应用	W/IP/IF-IC
目标/特异性	α-N-Catenin (C12G4) Rabbit mAb detects endogenous levels of total α-N-catenin protein. The antibody does not cross-react with other α-catenin family members.
使用方法	WB(1:1000) IP (1:250) IF-IC (1:50)

性能
供应商	CST
背景	Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1).   Recent evidence suggests that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4).
存放说明	-20C
计算分子量	102

参考图片

Immunoprecipitation of α-N-Catenin from SH-SY5Y cell lysates using α-N-Catenin (C12G4) Rabbit mAb. Western blot was performed using the same antibody.使用α-N-Catenin (C12G4) 兔单抗对SH-SY5Y细胞α-N-Catenin 进行免疫共产党。western blot使用同样的抗体。

Western blot analysis of extracts from SH-SY5Y cells, mouse brain and rat brain using α-N-Catenin (C12G4) Rabbit mAb.使用α-N-Catenin (C12G4) 兔单抗对SH-SY5Y细胞，小鼠大脑和大鼠大脑提取物进行western blot分析。

Confocal immunofluorescent analysis of SH-SY5Y cells using α-N-Catenin (C12G4) Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).使用α-N-Catenin (C12G4) 兔单抗（绿色）和S6核糖体蛋白（54D2）小鼠单抗#2317（红色）对SH-SY5Y激光共聚焦免疫荧光分析。蓝色假色=DRAQ5™（DNA荧光染料）。