| 货号 | 36611S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
Confocal immunofluorescent analysis of SK-OV-3 (left) and PC-3 (right) cells, using α-E-Catenin (D9R5E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from SK-OV-3 and PC-3 cells using α-E-catenin (D9R5E) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb (lower). | |
Western blot analysis of extracts from various cell lines using α-E-catenin (D9R5E) Rabbit mAb. |
