| 货号 | 2705S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/F |
| 目标/特异性 | Zap-70 (99F2) Rabbit mAb detects endogenous levels of total Zap-70. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) F (1:200) |
| 供应商 | CST |
| 背景 | The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 70 |
Western blot analysis of extracts from Jurkat cells, starved for 16 hours and either untreated or H2O2-treated (2 mM for 3 minutes), using Zap-70 (99F2) Rabbit mAb.Western免疫印迹。用Zap-70 (99F2) Rabbit mAb研究未经处理的和经H2O2(2 mM,3 min)处理的血清饥饿16小时的Jurkat细胞的细胞提取液。 | |
Flow cytometric analysis of Ramos B cells (blue) and Jurkat T cells (green), using Zap-70 (99F2) Rabbit mAb.流式细胞仪分析Ramos B (蓝色) 和Jurkat T (绿色)细胞，所用的抗体为Zap-70 (99F2) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded Jurkat T cells (left) and Ramos B cells (right), using Zap-70 (99F2) Rabbit mAb.免疫组织化学染色分析石蜡包埋Jurkat T 细胞 (左图) 和 Ramos B细胞(右图)。所用抗体为Zap-70 (99F2) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil, using Zap-70 (99F2) Rabbit mAb.免疫组织化学染色分析石蜡包埋人扁桃体组织。所用抗体为Zap-70 (99F2) Rabbit mAb。 |
